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Addgene

AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites
(Plasmid #120295)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120295 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZac2.1
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    C-terminal fragment of SpyCas9
  • Alt name
    AcrIIA4 with two miR-122 binding sites
  • Tag / Fusion Protein
    • split-intein (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites was a gift from Dominik Niopek (Addgene plasmid # 120295 ; http://n2t.net/addgene:120295 ; RRID:Addgene_120295)

  • For your References section:

    Cell-specific CRISPR-Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins. Hoffmann MD, Aschenbrenner S, Grosse S, Rapti K, Domenger C, Fakhiri J, Mastel M, Borner K, Eils R, Grimm D, Niopek D. Nucleic Acids Res. 2019 Apr 15. pii: 5458120. doi: 10.1093/nar/gkz271. 10.1093/nar/gkz271 PubMed 30982889
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