AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)
(Plasmid #120293)

• Purpose: AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold
• Depositing Lab: Dominik Niopek
• Publication: Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 5458120. doi: 10.1093/nar/gkz271.

Backbone

• Vector backbone: pZac2.1
• Vector type: Mammalian Expression, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: N-terminal fragment of SpyCas9
• Alt name: U6 scaffold
• Tags / Fusion Proteins:
  • SV40-NLS (N terminal on insert)
  • split-intein (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • 120293_AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint