pSYN-VWF-039
(Plasmid #120292)

• Purpose: VWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion protein
• Depositing Lab: Robert Peters
• Publication: Dong et al Blood. 2019 Jan 14. pii: blood-2018-10-876300. doi: 10.1182/blood-2018-10-876300.

Backbone

• Vector backbone: pcDNA4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5011
• Total vector size (bp): 9503
• Modifications to backbone: -
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VWF D1-D2-D'-D3-Fc
• Species: H. sapiens (human)
• Insert Size (bp): 4482
• Mutation: C1099A/C1142A
• Promoter: CMV
• Tag / Fusion Protein:
  • Fc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsiWI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry