pRK5 FLAG membrane-bound lipin-1 (TMD)
(Plasmid #120277)

• Purpose: Expression of ER/Golgi-tethered lipin-1 in mammalian cells
• Depositing Lab: Sirio Dupont
• Publication: Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. doi: 10.1038/s41556-018-0270-5. Epub 2019 Feb 4.

Backbone

• Vector backbone: pRK5
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lipin-1
• Alt name: phosphatidate phosphatase 1
• Alt name: fatty liver dystrophy
• Alt name: PAP1
• Species: M. musculus (mouse)
• Insert Size (bp): 2900
• Entrez Gene: Lpin1 (a.k.a. Lipin1, fld)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • in-frame fusion with the TMD Golgi-localization domain of Syntaxin5 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: David Sabatini Addgene Plasmid #32005

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry