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Purpose(Empty Backbone) Blunt-end cloning vector of PCR-products using ZERO-backgound system
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 120275 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC18
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin + 1% Glucose
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsneed to add glucose (1% final) to plates and media for optimal growth
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRZero was a gift from Ken Motohashi (Addgene plasmid # 120275 ; http://n2t.net/addgene:120275 ; RRID:Addgene_120275) -
For your References section:
A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Motohashi K. Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.1038/s41598-019-42868-6. 10.1038/s41598-019-42868-6 PubMed 31015513
Map uploaded by the depositor.
