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Addgene

013LP-pAAV-ANF-657-EmGFP-pA
(Plasmid #120267)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120267 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEMBL8
  • Backbone size w/o insert (bp) 2500
  • Total vector size (bp) 4709
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Confirm ITRs are not recombined with individual digests: XmaI, SnaBI, PvuII.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EmGFP
  • Alt name
    Emerald Green Fluorescent Protein
  • Species
    Aequorea Victoria
  • Insert Size (bp)
    766
  • Promoter ANF promoter
  • Tags / Fusion Proteins
    • 3XFlag (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    013LP-pAAV-ANF-657-EmGFP-pA was a gift from Xander Wehrens (Addgene plasmid # 120267 ; http://n2t.net/addgene:120267 ; RRID:Addgene_120267)

  • For your References section:

    Atrial-Specific Gene Delivery Using an Adeno-Associated Viral Vector. Ni L, Scott L Jr, Campbell HM, Pan X, Alsina KM, Reynolds J, Philippen LE, Hulsurkar M, Lagor WR, Li N, Wehrens XHT. Circ Res. 2019 Jan 18;124(2):256-262. doi: 10.1161/CIRCRESAHA.118.313811. 10.1161/CIRCRESAHA.118.313811 PubMed 30582449
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