pLV-EF1a-V5-LIC
(Plasmid
#120247)
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Purpose(Empty Backbone) For LIC cloning with N-terminal V5 tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 120247 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLV-EF1a-IRES-Puro
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Vector typeLentiviral
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Selectable markersPuromycin
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Tag
/ Fusion Protein
- V5 (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- V5 (N terminal on backbone)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For LIC cloning: cut with SrfI before T4 polymerase treatment
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-EF1a-V5-LIC was a gift from Larry Gerace (Addgene plasmid # 120247 ; http://n2t.net/addgene:120247 ; RRID:Addgene_120247) -
For your References section:
Identification of new transmembrane proteins concentrated at the nuclear envelope using organellar proteomics of mesenchymal cells. Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolome S, Tapia O, Zhang X, Yates JR 3rd, Gerace L. Nucleus. 2019 Dec;10(1):126-143. doi: 10.1080/19491034.2019.1618175. 10.1080/19491034.2019.1618175 PubMed 31142202
Map uploaded by the depositor.
