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Addgene

pLV-EF1a-V5-Sec61B
(Plasmid #120244)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120244 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-EF1a-IRES-Puro
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sec61B
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Sec61b (a.k.a. 1190006C12Rik, AI326121, AW122942)
  • Promoter EF1a
  • Tag / Fusion Protein
    • V5 (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-EF1a-V5-Sec61B was a gift from Larry Gerace (Addgene plasmid # 120244 ; http://n2t.net/addgene:120244 ; RRID:Addgene_120244)

  • For your References section:

    Identification of new transmembrane proteins concentrated at the nuclear envelope using organellar proteomics of mesenchymal cells. Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolome S, Tapia O, Zhang X, Yates JR 3rd, Gerace L. Nucleus. 2019 Dec;10(1):126-143. doi: 10.1080/19491034.2019.1618175. 10.1080/19491034.2019.1618175 PubMed 31142202
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