pLV-EF1a-V5-Smpd4
(Plasmid
#120237)
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PurposeExpresses V5-tagged Smpd4 in lentiviral vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 120237 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV-EF1a-IRES-Puro
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmpd4
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SpeciesM. musculus (mouse)
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Entrez GeneSmpd4 (a.k.a. 4122402O22Rik)
- Promoter EF1a
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Tag
/ Fusion Protein
- V5 (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-EF1a-V5-Smpd4 was a gift from Larry Gerace (Addgene plasmid # 120237 ; http://n2t.net/addgene:120237 ; RRID:Addgene_120237) -
For your References section:
Identification of new transmembrane proteins concentrated at the nuclear envelope using organellar proteomics of mesenchymal cells. Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolome S, Tapia O, Zhang X, Yates JR 3rd, Gerace L. Nucleus. 2019 Dec;10(1):126-143. doi: 10.1080/19491034.2019.1618175. 10.1080/19491034.2019.1618175 PubMed 31142202