pCas9CR5
(Plasmid #120227)

• Purpose: pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
• Depositing Lab: Christopher Reisch
• Publication: pCas9CR4 variants for increased targeting space (unpublished)

Backbone

• Vector backbone: pdCas9-bacteria
• Total vector size (bp): 6765
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SpCas9
• Species: Synthetic; E. coli
• Insert Size (bp): 4200
• Mutation: Stop codon inserted before ssrA degradation tag
• Promoter: pTet

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: gttgacactctatcgttgat

Resource Information

• Supplemental Documents:
  • pCas9CR5.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene, Stanley Qi

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry