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Addgene

pLentiCRISPRv1-CENPB
(Plasmid #120208)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120208 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLentiCRISPRv1
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CENPB poly(A) site
  • gRNA/shRNA sequence
    GTCACCCCCACAAAAGGGGAG
  • Species
    H. sapiens (human)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiCRISPRv1-CENPB was a gift from David Bartel (Addgene plasmid # 120208 ; http://n2t.net/addgene:120208 ; RRID:Addgene_120208)

  • For your References section:

    Widespread Influence of 3'-End Structures on Mammalian mRNA Processing and Stability. Wu X, Bartel DP. Cell. 2017 May 18;169(5):905-917.e11. doi: 10.1016/j.cell.2017.04.036. 10.1016/j.cell.2017.04.036 PubMed 28525757
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