GFP-SBP-VAP-A*
(Plasmid #120175)

• Purpose: Expresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-A
• Depositing Lab: Juan Bonifacino
• Publication: Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. doi: 10.1371/journal.pbio.3000279. eCollection 2019 May.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4721
• Total vector size (bp): 5237
• Modifications to backbone: Contains mutation A206K for producing monomeric eGFP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VAMP associated protein A
• Alt name: VAP-A
• Species: H. sapiens (human)
• Insert Size (bp): 516
• Mutation: Truncated VAP-A: 118-249
• Entrez Gene: VAPA (a.k.a. VAMP-A, VAP-33, VAP-A, VAP33, hVAP-33)
• Promoter: CMV
• Tags / Fusion Proteins:
  • EGFP (N terminal on backbone)
  • SBP (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: SV40pA rev
• 3′ sequencing primer: EGFPC1 fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry