TOM20*-SBP-GFP
(Plasmid #120173)

• Purpose: Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)
• Depositing Lab: Juan Bonifacino
• Publication: Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. doi: 10.1371/journal.pbio.3000279. eCollection 2019 May.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4715
• Total vector size (bp): 4916
• Modifications to backbone: Contains mutation A206K for producing monomeric eGFP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mitochondrial import receptor subunit TOM20 homolog
• Alt name: TOM20
• Species: H. sapiens (human)
• Insert Size (bp): 201
• Mutation: Truncated TOM20: 1-30 aa
• Entrez Gene: TOMM20 (a.k.a. MAS20, MOM19, TOM20)
• Promoter: CMV
• Tags / Fusion Proteins:
  • SBP (C terminal on insert)
  • eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: EGFPN1 rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry