LAMP1-SBP-GFP
(Plasmid #120172)

• Purpose: Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)
• Depositing Lab: Juan Bonifacino
• Publication: Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. doi: 10.1371/journal.pbio.3000279. eCollection 2019 May.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4692
• Total vector size (bp): 6074
• Modifications to backbone: Contains mutation A206K for producing monomeric eGFP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lysosomal-associated membrane protein 1
• Alt name: LAMP1
• Species: R. norvegicus (rat)
• Insert Size (bp): 1382
• Entrez Gene: Lamp1 (a.k.a. LGP120)
• Promoter: CMV
• Tags / Fusion Proteins:
  • SBP (C terminal on insert)
  • eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: EGFPN1 rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry