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Addgene

Strep-KIFC1*-HA
(Plasmid #120171)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120171 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3955
  • Total vector size (bp) 6175
  • Modifications to backbone
    Replaced the mCherry for an HA-tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kinesin family member C1
  • Alt name
    KIFC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2220
  • Mutation
    Truncated KIFC1: 125-673 aa
  • GenBank ID
    NM_002263
  • Entrez Gene
    KIFC1 (a.k.a. HSET, KNSL2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Streptavidin (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV fwd
  • 3′ sequencing primer SV40pA rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Strep-KIFC1*-HA was a gift from Juan Bonifacino (Addgene plasmid # 120171 ; http://n2t.net/addgene:120171 ; RRID:Addgene_120171)

  • For your References section:

    Reversible association with motor proteins (RAMP): A streptavidin-based method to manipulate organelle positioning. Guardia CM, De Pace R, Sen A, Saric A, Jarnik M, Kolin DA, Kunwar A, Bonifacino JS. PLoS Biol. 2019 May 17;17(5):e3000279. doi: 10.1371/journal.pbio.3000279. eCollection 2019 May. 10.1371/journal.pbio.3000279 PubMed 31100061
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