Strep-KIFC1*-mCh
(Plasmid #120169)

• Purpose: Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)
• Depositing Lab: Juan Bonifacino
• Publication: Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. doi: 10.1371/journal.pbio.3000279. eCollection 2019 May.

Backbone

• Vector backbone: pmCherry-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4711
• Total vector size (bp): 6856
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: kinesin family member C1
• Alt name: KIFC1
• Species: H. sapiens (human)
• Insert Size (bp): 2145
• Mutation: Truncated KIFC1: 125-673 aa
• GenBank ID: NM_002263
• Entrez Gene: KIFC1 (a.k.a. HSET, KNSL2)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Streptavidin (N terminal on insert)
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: mChN1 rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry