mCh-KIF5A*-strep
(Plasmid #120163)

• Purpose: Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin
• Depositing Lab: Juan Bonifacino
• Publication: Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. doi: 10.1371/journal.pbio.3000279. eCollection 2019 May.

Backbone

• Vector backbone: pmCherry-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4727
• Total vector size (bp): 6902
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: kinesin family member 5A
• Alt name: KIF5A
• Species: M. musculus (mouse)
• Insert Size (bp): 2175
• Mutation: Truncated KIF5A: 1-559 aa
• Entrez Gene: Kif5a (a.k.a. D10Bwg0738e, Khc, Kif5, Kns, mKIAA4086)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mCherry (N terminal on backbone)
  • Streptavidin (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: mChC1 fwd
• 3′ sequencing primer: SV40pA rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry