pCAGGS-FuG-E (P440E)
(Plasmid
#119978)
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PurposeThis is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E (P440E) envelope coating virus particle with NeuRet.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119978 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAGGS vector
- Backbone size w/o insert (bp) 4900
- Total vector size (bp) 6406
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsStbl3 and DH5alpha are also suitable growth strains.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFusion Glycoprotein type E (P440E)
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Alt nameFuG-E (P440E)
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SpeciesG. gallus (chicken), B. taurus (bovine); Vesicular stomatitis Indiana virus, Rabies virus, Cytomegalovirus, Simian virus 40, Oryctolagus cuniculus
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Insert Size (bp)1515
- Promoter CAGGS
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Tag
/ Fusion Protein
- Fusion protein of Vesicular stomatitis Indiana virus and Rabies virus (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown
- 3′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS was unable to fully resolve the sequence of the CAGGS promoter. Please refer to the depositor's sequence for this section of the plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-FuG-E (P440E) was a gift from Kazuto Kobayashi (Addgene plasmid # 119978 ; http://n2t.net/addgene:119978 ; RRID:Addgene_119978) -
For your References section:
Enhancement of the transduction efficiency of a lentiviral vector for neuron-specific retrograde gene delivery through the point mutation of fusion glycoprotein type E. Kato S, Sugawara M, Kobayashi K, Kimura K, Inoue KI, Takada M, Kobayashi K. J Neurosci Methods. 2018 Oct 19;311:147-155. doi: 10.1016/j.jneumeth.2018.10.023. 10.1016/j.jneumeth.2018.10.023 PubMed 30347222
Map uploaded by the depositor.
