pET28GSTlinker-LIC
(Plasmid
#119970)
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Purpose(Empty Backbone) Structure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119970 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SGC vector. Cloning Site 5-prime': TTGTATTTCCAGGGC; Cloning Site 3-prime: CAAGCTTCGTCATCA,
SGC link: https://www.thesgc.org/reagents/vectors
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28GSTlinker-LIC was a gift from Cheryl Arrowsmith (Addgene plasmid # 119970 ; http://n2t.net/addgene:119970 ; RRID:Addgene_119970)