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Addgene

pGL3-Basic-OPN-600
(Plasmid #11997)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11997 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Osteopontin
  • Alt name
    OPN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Mutation
    The following construct contains the OPN promoter, which drives the expression of luciferase. This construct contains 600 bp of the OPN promoter.
  • GenBank ID
    NT_016354
  • Entrez Gene
    SPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site nhe1 (not destroyed)
  • 3′ cloning site xho1 (not destroyed)
  • 5′ sequencing primer rvprimer3
  • 3′ sequencing primer glprimer2
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-Basic-OPN-600 was a gift from Steven Johnson (Addgene plasmid # 11997 ; http://n2t.net/addgene:11997 ; RRID:Addgene_11997)
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