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Addgene

6CUI (BRD2)
(Plasmid #119963)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119963 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28GSTlinker-LIC
  • Backbone manufacturer
    SGC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BRD2
  • Alt name
    PDB ID: 6CUI
  • Alt name
    BRD2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    348
  • Entrez Gene
    BRD2 (a.k.a. BRD2-IT1, D6S113E, FSH, FSHRG1, FSRG1, NAT, O27.1.1, RING3, RNF3)
  • Promoter T7
  • Tag / Fusion Protein
    • GST tag followed by a long linker and TEV cleavage site (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N-Tag: MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSSEGDATMGHMVHRPFQSAASYPNRPSKTPPRAAESSGRENLYFQG. BRD2:JMC137-F11:C241626. SGC link: http://www.thesgc.org/structures/6CUI/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6CUI (BRD2) was a gift from Cheryl Arrowsmith (Addgene plasmid # 119963 ; http://n2t.net/addgene:119963 ; RRID:Addgene_119963)
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