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Addgene

6CKO (DOT1L)
(Plasmid #119962)

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Item Catalog # Description Quantity Price (USD)
Plasmid 119962 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET28-MKH8SUMO
  • Backbone manufacturer
    SGC (Addgene Plasmid #79526)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DOT1L
  • Alt name
    PDB ID: 6CKO
  • Alt name
    Dot1L;gi|163965372
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    147
  • Entrez Gene
    DOT1L (a.k.a. DOT1, KMT4)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHIS, thrombin,SUMO and TEV cleavage (N terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N-Tag: MKKHHHHHHHHSSGLVPRGSMSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGGTENLYFQG. DOT1L:JMC107-B04:C239899. SGC link: http://www.thesgc.org/structures/6CKO/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6CKO (DOT1L) was a gift from Cheryl Arrowsmith (Addgene plasmid # 119962 ; http://n2t.net/addgene:119962 ; RRID:Addgene_119962)
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