6CDG (GID4)
(Plasmid
#119961)
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PurposeBacterial expression for structure determination
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119961 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepET28-MHL
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Backbone manufacturerSGC (Addgene Plasmid #26096)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGID4
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Alt namePDB ID: 6CDG, 6WZZ, 7S12, 7SLZ
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Alt nameBC041829
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SpeciesH. sapiens (human)
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Insert Size (bp)498
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Mutationcontains amino acids 124-289
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Entrez GeneC17orf39 (a.k.a. MGC3048)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis-TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
N-Tag:MHHHHHHSSGRENLYFQG. GID4:JMC130-A04:C240838.
SGC links:
https://www.thesgc.org/structures/6CDG/
https://www.thesgc.org/structures/6WZZ/
https://www.thesgc.org/structures/7S12/
https://www.thesgc.org/structures/7SLZ/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
6CDG (GID4) was a gift from Cheryl Arrowsmith (Addgene plasmid # 119961 ; http://n2t.net/addgene:119961 ; RRID:Addgene_119961)