6BHD (SETDB1)
(Plasmid
#119955)
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PurposeBacterial expression for structure determination
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119955 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET28-MHL
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Backbone manufacturerSGC (Addgene Plasmid #26096)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSETDB1
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Alt namePDB ID: 6BHD, 6BHE, 6BHG, 6BHH and 6BHI
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Alt nameBC028671
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SpeciesH. sapiens (human)
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Insert Size (bp)663
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Mutationcontains amino acids 190-410
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Entrez GeneSETDB1 (a.k.a. ESET, H3-K9-HMTase4, KG1T, KMT1E, TDRD21)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis-TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
N-Tag:MHHHHHHSSGRENLYFQG. SETDB1:JMC001-H06:C41642. SGC links: http://www.thesgc.org/structures/6BHD/, http://www.thesgc.org/structures/6BHE/, http://www.thesgc.org/structures/6BHG/, http://www.thesgc.org/structures/6BHH/, http://www.thesgc.org/structures/6BHI/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
6BHD (SETDB1) was a gift from Cheryl Arrowsmith (Addgene plasmid # 119955 ; http://n2t.net/addgene:119955 ; RRID:Addgene_119955)
