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Addgene

pGL403
(Plasmid #119953)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119953 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBbB2a-GFP
  • Backbone manufacturer
    Lee, Taek Soon, et al. "BglBrick vectors and datasheets: a synthetic biology platform for gene expression." Journal of biologica
  • Total vector size (bp) 5880
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    gpps
  • Species
    Abies grandis
  • Mutation
    N-terminal truncation of 85 amino acid residues (signal sequence)

Gene/Insert 2

  • Gene/Insert name
    limS
  • Species
    Mentha spicata
  • Mutation
    N-terminal truncation of 56 amino acid residues (signal sequence)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL403 was a gift from Nigel Scrutton (Addgene plasmid # 119953 ; http://n2t.net/addgene:119953 ; RRID:Addgene_119953)

  • For your References section:

    Machine learning of designed translational control allows predictive pathway optimisation in Escherichia coli. Jervis AJ, Carbonell P, Vinaixa M, Dunstan MS, Hollywood KA, Robinson CJ, Rattray NJW, Yan C, Swainston N, Currin A, Sung R, Toogood HS, Taylor S, Faulon JL, Breitling R, Takano E, Scrutton NS. ACS Synth Biol. 2018 Dec 18. doi: 10.1021/acssynbio.8b00398. 10.1021/acssynbio.8b00398 PubMed 30563328
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