pMVA2
(Plasmid
#119951)
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PurposeExpresses recombinant MVA pathway in Escherichia coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119951 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBbA1k-RFP
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Backbone manufacturerLee, Taek Soon, et al. "BglBrick vectors and datasheets: a synthetic biology platform for gene expression." Journal of biologica
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namemvaE
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SpeciesEnterococcus faecalis
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Insert Size (bp)2412
Gene/Insert 2
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Gene/Insert namemvaS
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SpeciesEnterococcus faecalis
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MutationA110G
Gene/Insert 3
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Gene/Insert namemvaK1
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SpeciesStreptococcus pneumoniae
Gene/Insert 4
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Gene/Insert namemvaK2
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SpeciesStreptococcus pneumoniae
Gene/Insert 5
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Gene/Insert namemvaD
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SpeciesStreptococcus pneumoniae
Gene/Insert 6
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Gene/Insert nameidi
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SpeciesEscherichia coli
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMVA2 was a gift from Nigel Scrutton (Addgene plasmid # 119951 ; http://n2t.net/addgene:119951 ; RRID:Addgene_119951) -
For your References section:
Machine learning of designed translational control allows predictive pathway optimisation in Escherichia coli. Jervis AJ, Carbonell P, Vinaixa M, Dunstan MS, Hollywood KA, Robinson CJ, Rattray NJW, Yan C, Swainston N, Currin A, Sung R, Toogood HS, Taylor S, Faulon JL, Breitling R, Takano E, Scrutton NS. ACS Synth Biol. 2018 Dec 18. doi: 10.1021/acssynbio.8b00398. 10.1021/acssynbio.8b00398 PubMed 30563328