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PurposeLentiviral vector expressing G3BP1-SNAP fusion protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119949 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHAGE-UbiC
- Backbone size w/o insert (bp) 7000
- Total vector size (bp) 8219
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Vector typeMammalian Expression, Bacterial Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameG3BP1
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SpeciesH. sapiens (human)
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Insert Size (bp)1398
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Entrez GeneG3BP1 (a.k.a. G3BP, HDH-VIII)
- Promoter UbiC
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Tag
/ Fusion Protein
- SNAP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GTGTGGCACAGCTAGTTCC (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/332502v1 for BioRxiv preprint
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
phage UbiC G3BP1-SNAP was a gift from Jeffrey Chao (Addgene plasmid # 119949 ; http://n2t.net/addgene:119949 ; RRID:Addgene_119949) -
For your References section:
Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response. Wilbertz JH, Voigt F, Horvathova I, Roth G, Zhan Y, Chao JA. Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 10.1016/j.molcel.2018.12.006. 10.1016/j.molcel.2018.12.006 PubMed 30661979
