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phage UbiC SNAP-DDX6
(Plasmid #119948)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119948 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE-UbiC
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 8270
  • Vector type
    Mammalian Expression, Bacterial Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDX6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1446
  • Entrez Gene
    DDX6 (a.k.a. HLR2, IDDILF, P54, RCK, Rck/p54)
  • Promoter UbiC
  • Tag / Fusion Protein
    • SNAP (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/332502v1 for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phage UbiC SNAP-DDX6 was a gift from Jeffrey Chao (Addgene plasmid # 119948 ; http://n2t.net/addgene:119948 ; RRID:Addgene_119948)

  • For your References section:

    Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response. Wilbertz JH, Voigt F, Horvathova I, Roth G, Zhan Y, Chao JA. Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 10.1016/j.molcel.2018.12.006. 10.1016/j.molcel.2018.12.006 PubMed 30661979
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