pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
(Plasmid #119946)

• Purpose: Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
• Depositing Lab: Jeffrey Chao
• Publication: Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 10.1016/j.molcel.2018.12.006.

Backbone

• Vector backbone: pSF4
• Backbone size w/o insert (bp): 10500
• Total vector size (bp): 11304
• Vector type: Mammalian Expression, Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Renilla luciferase
• Species: Renilla reniformis
• Insert Size (bp): 930
• Promoter: TetCMV
• Tags / Fusion Proteins:
  • 5'TOP sequence
  • 24xGCN4 repeats (SunTag) (N terminal on insert)
  • FKBP (C terminal on insert)
  • 24xMS2v5 stem loops in 3'UTR

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: AATACGACTCACTATAGGC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/332502v1 for BioRxiv preprint

Note plasmid contains 24xGCN4 repeats and not 20xGCN4 as described in the name.