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PurposeExpress TS2126 RNA ligase in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119941 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5357
- Total vector size (bp) 6557
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTS2126 RNA ligase
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SpeciesPhage TS2126
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Insert Size (bp)1200
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GenBank IDCQ796011.1
- Promoter T7
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Tag
/ Fusion Protein
- His6 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pET28a-F
- 3′ sequencing primer pET28a-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-TS2126 RNA Ligase was a gift from Takashi Ito (Addgene plasmid # 119941 ; http://n2t.net/addgene:119941 ; RRID:Addgene_119941) -
For your References section:
Highly efficient single-stranded DNA ligation technique improves low-input whole-genome bisulfite sequencing by post-bisulfite adaptor tagging. Miura F, Shibata Y, Miura M, Sangatsuda Y, Hisano O, Araki H, Ito T. Nucleic Acids Res. 2019 May 22. pii: 5494777. doi: 10.1093/nar/gkz435. 10.1093/nar/gkz435 PubMed 31114914
Map uploaded by the depositor.
