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Addgene

pGL3-Basic-Pax8
(Plasmid #11994)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11994 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PAX8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2300
  • Mutation
    The following construct contains the PAX8 promoter, which drives the expression of luciferase. This construct contains 1300 bp of the PAX8 promoter.
  • GenBank ID
    AB062505
  • Entrez Gene
    PAX8 (a.k.a. PAX-8)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer rvprimer3
  • 3′ sequencing primer glprimer2
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-Basic-Pax8 was a gift from Steven Johnson (Addgene plasmid # 11994 ; http://n2t.net/addgene:11994 ; RRID:Addgene_11994)
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