HA-RNF34
(Plasmid #119938)

• Purpose: RING finger protein 34 expression (HA-tag at the N-terminus)
• Depositing Lab: Angel de Blas
• Publication: Jin et al J Biol Chem. 2014 Oct 17;289(42):29420-36. doi: 10.1074/jbc.M114.603068. Epub 2014 Sep 5.

Backbone

• Vector backbone: pCAGGS
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RNF34
• Alt name: Momo
• Species: R. norvegicus (rat)
• Entrez Gene: Rnf34 (a.k.a. Momo)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: BgIII (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Insert was originally cloned in Angel de Blas's lab.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry