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Addgene

RNF34
(Plasmid #119937)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119937 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNF34
  • Alt name
    Momo
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Rnf34 (a.k.a. Momo)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BgIII (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert was originally cloned in Angel de Blas's lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RNF34 was a gift from Angel de Blas (Addgene plasmid # 119937 ; http://n2t.net/addgene:119937 ; RRID:Addgene_119937)

  • For your References section:

    Ring finger protein 34 (RNF34) interacts with and promotes gamma-aminobutyric acid type-A receptor degradation via ubiquitination of the gamma2 subunit. Jin H, Chiou TT, Serwanski DR, Miralles CP, Pinal N, De Blas AL. J Biol Chem. 2014 Oct 17;289(42):29420-36. doi: 10.1074/jbc.M114.603068. Epub 2014 Sep 5. 10.1074/jbc.M114.603068 PubMed 25193658
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