Myc-PCDHGC5
(Plasmid #119935)

• Purpose: Protocadherin gammaC5 expression (Myc-tag is at the linker between EC1 and EC2 extracellular cadherin repeats)
• Depositing Lab: Angel de Blas
• Publication: Li et al J Neurosci. 2012 Aug 22;32(34):11780-97. doi: 10.1523/JNEUROSCI.0969-12.2012.

Backbone

• Vector backbone: pCDNA3.1(+)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PCDHGC5
• Species: R. norvegicus (rat)
• Entrez Gene: Pcdhgc5
• Tag / Fusion Protein:
  • 9E10 cMyc epitope (EQKLISEEDL) was inserted between amino acids 103 and 104 of mature protein (after signal peptide cleavage) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Insert was originally cloned in Angel de Blas's lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry