pYM70
(Plasmid #119922)

• Purpose: CaHygB plasmids that facilitate genetic manipulations in the fungal pathogen
• Depositing Lab: Brian Wong
• Publication: Basso LR et al Yeast. 2010 Dec;27(12):1039-48. doi: 10.1002/yea.1813. Epub 2010 Aug 24.

Backbone

• Vector backbone: pUC18
• Backbone size w/o insert (bp): 6299
• Total vector size (bp): 7331
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CaHygB
• Alt name: hygromycin B resistance gene
• Species: Candida albicnas
• Insert Size (bp): 1032
• Promoter: TEF2
• Tag / Fusion Protein:
  • CaHygB (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AatII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CTCGAGATGAAAAAACCAGAATTGACTGC
• 3′ sequencing primer: GAGCTAAAGAATAAGGATCC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Luiz Basso, Charlie Gast
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry