pIND-HA-ADH
(Plasmid
#11991)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11991 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIND
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression ; Ecdysone Inducible
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namealdehyde dehydrogenase
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Alt nameALDH1
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SpeciesH. sapiens (human)
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Insert Size (bp)1505
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Mutationno mutations
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GenBank IDNM_000689
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Entrez GeneALDH1A1 (a.k.a. ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1)
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer see pIND
- 3′ sequencing primer see pIND (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is a GFP inducible gene construct....inducible with pronosterone or other analogs.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIND-HA-ADH was a gift from Steven Johnson (Addgene plasmid # 11991 ; http://n2t.net/addgene:11991 ; RRID:Addgene_11991)