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epB_CAG_TetRFlag-nls-tdTom_DEx4
(Plasmid #119909)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119909 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ePiggyBac-CAG
  • Backbone size w/o insert (bp) 5670
  • Total vector size (bp) 7827
  • Modifications to backbone
    4x RNA destabilizing element (UUAUUUAUU) in 3'UTR
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TetRFlag-nls-tdTom
  • Species
    Synthetic

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid contains ICP22 NLS with a single aa change that does not affect function. Sequence: RRPAVRSPPLGTRKRK.

Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    epB_CAG_TetRFlag-nls-tdTom_DEx4 was a gift from Orion Weiner (Addgene plasmid # 119909 ; http://n2t.net/addgene:119909 ; RRID:Addgene_119909)

  • For your References section:

    Live-cell imaging reveals enhancer-dependent Sox2 transcription in the absence of enhancer proximity. Alexander JM, Guan J, Li B, Maliskova L, Song M, Shen Y, Huang B, Lomvardas S, Weiner OD. Elife. 2019 May 24;8. pii: 41769. doi: 10.7554/eLife.41769. 10.7554/eLife.41769 PubMed 31124784
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