pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP
(Plasmid #119903)

• Purpose: Bxb1 integration plasmid for cuO array with Puro selection cassette
• Depositing Lab: Orion Weiner
• Publication: Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 10.7554/eLife.41769.

Backbone

• Vector backbone: pDEST-Bxb1attB-loxP-PGK-puro-loxP
• Backbone size w/o insert (bp): 4904
• Total vector size (bp): 10125
• Vector type: Mouse Targeting
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: This plasmid is unstable due to repetitive elements. Please screen 3-5 colonies to ensure the full plasmid is selected. See comments section for more details.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cuOx144 array
• Species: Synthetic
• Insert Size (bp): 5221

Resource Information

• Supplemental Documents:
  • 286_pDEST_cuOx144_Bxb1_attB_loxP_PGKpuro_loxP.gb
  • cuO and tetO array genome integration protocol
• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note there are a few ambiguous bases in Addgene's NGS result. This is due to NGS having difficulty assembling repetitive sequences.

The repetitive sequences in this plasmid can be unstable while propagated in bacteria. It is recommended that the repetitive insert be verified by restriction digest and repeat counts be viewed as an approximation.

Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.