Skip to main content
Addgene

pDEST-tetOx224_PhiC31attB_FRT-PGKneo-FRT
(Plasmid #119902)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119902 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDEST-PhiC31attB-FRT-PGK-neo-FRT
  • Backbone size w/o insert (bp) 5464
  • Total vector size (bp) 14953
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tetOx224 array
  • Species
    Synthetic
  • Insert Size (bp)
    9489

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PhiC31 integrase expression plasmid available on addGene (pPGKPhiC31obpA: #13795).

The repetitive sequences in this plasmid can be unstable while propagated in bacteria. It is recommended that the repetitive insert be verified by restriction digest and repeat counts be viewed as an approximation.

Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-tetOx224_PhiC31attB_FRT-PGKneo-FRT was a gift from Orion Weiner (Addgene plasmid # 119902)

  • For your References section:

    Live-cell imaging reveals enhancer-dependent Sox2 transcription in the absence of enhancer proximity. Alexander JM, Guan J, Li B, Maliskova L, Song M, Shen Y, Huang B, Lomvardas S, Weiner OD. Elife. 2019 May 24;8. pii: 41769. doi: 10.7554/eLife.41769. 10.7554/eLife.41769 PubMed 31124784
Related items: