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PurposeExpresses Bxb1 integrase in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119901 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAGGs
- Backbone size w/o insert (bp) 4905
- Total vector size (bp) 6450
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMammalian codon optimized Bxb1 integrase
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SpeciesMycobacteriophage Bxb1
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Insert Size (bp)1545
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-Bxb1o_nlsFlag was a gift from Orion Weiner (Addgene plasmid # 119901 ; http://n2t.net/addgene:119901 ; RRID:Addgene_119901) -
For your References section:
Live-cell imaging reveals enhancer-dependent Sox2 transcription in the absence of enhancer proximity. Alexander JM, Guan J, Li B, Maliskova L, Song M, Shen Y, Huang B, Lomvardas S, Weiner OD. Elife. 2019 May 24;8. pii: 41769. doi: 10.7554/eLife.41769. 10.7554/eLife.41769 PubMed 31124784