pBS C-ret
(Plasmid
#119900)
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PurposeMouse Ret In situ hybridization probe
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119900 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBS
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Vector typein situ hybridization probe
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameret
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Alt namec-Ret
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SpeciesM. musculus (mouse)
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Entrez GeneRet (a.k.a. PTC, RET51, RET9, c-Ret)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid can be used to generate an in situ hybridization antisense probe for mouse C-ret. Linearize the plasmid with NotI and use T7 to transcribe the ~1kb probe.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS C-ret was a gift from David Ginty & Wenqin Luo (Addgene plasmid # 119900 ; http://n2t.net/addgene:119900 ; RRID:Addgene_119900) -
For your References section:
Molecular identification of rapidly adapting mechanoreceptors and their developmental dependence on ret signaling. Luo W, Enomoto H, Rice FL, Milbrandt J, Ginty DD. Neuron. 2009 Dec 24;64(6):841-56. doi: 10.1016/j.neuron.2009.11.003. 10.1016/j.neuron.2009.11.003 PubMed 20064391