pCR2.1 GFR alpha 2
(Plasmid
#119899)
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PurposeMouse GFR alpha 2 In situ hybridization probe
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119899 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR2.1
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Backbone manufacturerInvitrogen
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Vector typein situ hybridization probe
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGDNF family receptor alpha-2
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Alt nameGfra2
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SpeciesM. musculus (mouse)
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Entrez GeneGfra2
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-rev
- 3′ sequencing primer M13-fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid can be used to generate an in situ hybridization antisense probe for mouse Gfr-alpha2. Acc65I or SpeI can be used to linearize the plasmid followed by SP6 to transcribe the ~1.5kb probe.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR2.1 GFR alpha 2 was a gift from David Ginty & Wenqin Luo (Addgene plasmid # 119899 ; http://n2t.net/addgene:119899 ; RRID:Addgene_119899) -
For your References section:
Molecular identification of rapidly adapting mechanoreceptors and their developmental dependence on ret signaling. Luo W, Enomoto H, Rice FL, Milbrandt J, Ginty DD. Neuron. 2009 Dec 24;64(6):841-56. doi: 10.1016/j.neuron.2009.11.003. 10.1016/j.neuron.2009.11.003 PubMed 20064391
