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pCRII-TOPO GFR alpha 1
(Plasmid #119898)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119898 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII-TOPO
  • Backbone manufacturer
    Invitrogen
  • Vector type
    in situ hybridization probe

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GDNF family receptor alpha-1 precursor
  • Alt name
    Gfra1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Gfra1 (a.k.a. GFRalpha-1)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid can be used to generate an in situ hybridization antisense probe for mouse Gfr-alpha1, using SP6 to transcribe the ~1.5kb probe.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRII-TOPO GFR alpha 1 was a gift from David Ginty & Wenqin Luo (Addgene plasmid # 119898 ; http://n2t.net/addgene:119898 ; RRID:Addgene_119898)

  • For your References section:

    Molecular identification of rapidly adapting mechanoreceptors and their developmental dependence on ret signaling. Luo W, Enomoto H, Rice FL, Milbrandt J, Ginty DD. Neuron. 2009 Dec 24;64(6):841-56. doi: 10.1016/j.neuron.2009.11.003. 10.1016/j.neuron.2009.11.003 PubMed 20064391
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