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Addgene

pGEMT-GDNF
(Plasmid #119897)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119897 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-T
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    in situ hybridization probe

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    glial cell line derived neurotrophic factor
  • Alt name
    Gdnf
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Gdnf (a.k.a. ATF)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid can be used to generate an in situ hybridization antisense probe for mouse GDNF, using SP6 to transcribe the ~1kb probe.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEMT-GDNF was a gift from Wenqin Luo (Addgene plasmid # 119897 ; http://n2t.net/addgene:119897 ; RRID:Addgene_119897)

  • For your References section:

    Cis and trans RET signaling control the survival and central projection growth of rapidly adapting mechanoreceptors. Fleming MS, Vysochan A, Paixao S, Niu J, Klein R, Savitt JM, Luo W. Elife. 2015 Apr 2;4:e06828. doi: 10.7554/eLife.06828. 10.7554/eLife.06828 PubMed 25838128
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