pGEMT-NRTN
(Plasmid
#119896)
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PurposeMouse NRTN In situ hybridization probe
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119896 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-T
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
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Vector typein situ hybridization probe
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameneurturin probe
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Alt nameNrtn
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SpeciesM. musculus (mouse)
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Entrez GeneNrtn (a.k.a. NTN)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid can be used to generate an in situ hybridization antisense probe for mouse NRTN, using T7 to transcribe the ~1kb probe.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEMT-NRTN was a gift from Wenqin Luo (Addgene plasmid # 119896 ; http://n2t.net/addgene:119896 ; RRID:Addgene_119896) -
For your References section:
Cis and trans RET signaling control the survival and central projection growth of rapidly adapting mechanoreceptors. Fleming MS, Vysochan A, Paixao S, Niu J, Klein R, Savitt JM, Luo W. Elife. 2015 Apr 2;4:e06828. doi: 10.7554/eLife.06828. 10.7554/eLife.06828 PubMed 25838128