pDEST-Hemmar-FLP(D)
(Plasmid
#119895)
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PurposeFLP(D) with a hsp70 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119895 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST-HemmarG
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Backbone manufacturerAddgene
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Vector typeDrosophila expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFLP(D)
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SpeciesD. melanogaster (fly)
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Mutationmutated form of FLP, which at position 5 contains an aspartic acid instead of glycine residue
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/06/07/341560 for BioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST-Hemmar-FLP(D) was a gift from Rita Sousa-Nunes (Addgene plasmid # 119895 ; http://n2t.net/addgene:119895 ; RRID:Addgene_119895) -
For your References section:
Spatiotemporally controlled genetic perturbation for efficient large-scale studies of cell non-autonomous effects. Chai A, Mateus AM, Oozeer F, Sousa-Nunes R. Elife. 2018 Nov 27;7. pii: 38393. doi: 10.7554/eLife.38393. 10.7554/eLife.38393 PubMed 30479273