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pDEST-Hemmar-DSCP-ftz-FLP(D)
(Plasmid #119894)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119894 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST-HemmarG
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 14231
  • Total vector size (bp) 13264
  • Vector type
    Drosophila expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    FLP(D)
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1268
  • Mutation
    a mutated form of FLP, which at position 5 contains an aspartic acid instead of glycine residue

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gac ctg cag gCT CGA GAA TCA AAA TGC C
  • 3′ sequencing primer act ggc tta gtt aat taa ttc tag atc tag att aAA TAC GGC GAT TGA TGT AG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    DSCP-ftz
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    146
  • Entrez Gene
    ftz (a.k.a. Dmel_CG2047, BG:DS07876.1, CG2047, Dm-Ftz, Dm-ftz, DmFtz, Dmel\CG2047, Dmftz, FTZ, Ftz, Ual, l(3)84Ag)

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tcg ttc agc tGA ATT GGC CGC GTT TAA AC
  • 3′ sequencing primer gat tct cga gCC TGC AGG TCT TTG CAA TC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-Hemmar-DSCP-ftz-FLP(D) was a gift from Rita Sousa-Nunes (Addgene plasmid # 119894 ; http://n2t.net/addgene:119894 ; RRID:Addgene_119894)

  • For your References section:

    Spatiotemporally controlled genetic perturbation for efficient large-scale studies of cell non-autonomous effects. Chai A, Mateus AM, Oozeer F, Sousa-Nunes R. Elife. 2018 Nov 27;7. pii: 38393. doi: 10.7554/eLife.38393. 10.7554/eLife.38393 PubMed 30479273
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