pDD87
(Plasmid
#119892)
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PurposeThe pLPD03031 promoter from Rhodococcus opacus PD630 expressing GFP+ on the pAL5000/pMB1 backbone with kanamycin resistance.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 119892 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAL5000/pMB1 shuttle vector
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDD87 was a gift from Tae Seok Moon (Addgene plasmid # 119892 ; http://n2t.net/addgene:119892 ; RRID:Addgene_119892) -
For your References section:
Development of Chemical and Metabolite Sensors for Rhodococcus opacus PD630. DeLorenzo DM, Henson WR, Moon TS. ACS Synth Biol. 2017 Oct 20;6(10):1973-1978. doi: 10.1021/acssynbio.7b00192. Epub 2017 Aug 10. 10.1021/acssynbio.7b00192 PubMed 28745867