pDD132
(Plasmid
#119885)
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PurposeIntegration vector into the ROCI-3 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119885 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep15a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDD132 was a gift from Tae Seok Moon (Addgene plasmid # 119885 ; http://n2t.net/addgene:119885 ; RRID:Addgene_119885) -
For your References section:
Molecular Toolkit for Gene Expression Control and Genome Modification in Rhodococcus opacus PD630. DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS. ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 10.1021/acssynbio.7b00416. Epub 2018 Feb 5. 10.1021/acssynbio.7b00416 PubMed 29366319
Map uploaded by the depositor.
