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PurposeArabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119880 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAL5000/pMB1 shuttle vector
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepBAD_dCas9sth1
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bydCas9sth1 received from Sarah Fortune (published as PMID: 28165460 )
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDD143 was a gift from Tae Seok Moon (Addgene plasmid # 119880 ; http://n2t.net/addgene:119880 ; RRID:Addgene_119880) -
For your References section:
Molecular Toolkit for Gene Expression Control and Genome Modification in Rhodococcus opacus PD630. DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS. ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 10.1021/acssynbio.7b00416. Epub 2018 Feb 5. 10.1021/acssynbio.7b00416 PubMed 29366319